Composite

Part:BBa_K4807022:Design

Designed by: Vaios Styliaras, Efi Chatzopoulou   Group: iGEM23_IOANNINA   (2023-10-11)


Catalase insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 248
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 248
    Illegal BamHI site found at 60
    Illegal BamHI site found at 513
    Illegal BamHI site found at 576
    Illegal XhoI site found at 1187
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 248
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 248
    Illegal NgoMIV site found at 1435
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1528


Design Notes

Minor base modifications have been made for proper Primer Design, for keeping translational frame intact etc


Source

Human Catalase gene comes from the Human Genome.

References